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Be effective in vivo, we first performed an antiproliferative screen to identify compound combinations that selectively kill, or prevent the proliferation, of human tumor cells in vitro. Using our cHTS method vide supra ; , we tested 100, 000 combinations from a set of 600 approved drugs. We identified and confirmed 13 synergistic combinations, after eliminating 28 synergistic combinations in which both compounds were known antineoplastics drugs. One of these combinations, comprising the antipsychotic agent chlorpromazine and the antiprotozoal agent pentamidine, was selected for further testing in vivo. Both of these compounds have moderate antiproliferative activities on their own in vitro in A549 lung carcinoma cells Fig. 4 AC ; , but neither demonstrates substantial activity at concentrations that would be considered clinically relevant in vitro or in vivo, and neither is currently used as an anticancer drug Fig. 4 AC ; . combination, however, these compounds prevented the growth of A549 lung carcinoma cells in vitro Fig. 4 AC ; and in vivo in a human tumor xenograft assay in mice Fig. 4D ; . The combination was even more effective than paclitaxel, a clinically used anticancer drug, at concentrations of the two drugs that correspond to clinically achievable plasma concentrations Fig. 4D ; . This growth inhibition occurred without loss of body weight or other visible behavior changes characteristic of mice and theophylline.
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382. Reducing musculoskeletal burden through ergonomic program implementation in a large newspaper - Cole D.C., Hogg-Johnson S., Manno M. et al. [D.C. Cole, Institute for Work and Health, 481 University Avenue, Toronto, ON M5G 2E9, Canada] - INT. ARCH. OCCUP. ENVIRON. HEALTH 2006 80 2 ; - summ in ENGL Objectives: To assess the impact of a workplace ergonomic program to reduce musculoskeletal burden among newspaper employees and to understand relationships among participation, risk factor changes and health status within an employee cohort. Methods: We conducted repeat cross-sectional surveys, with 1, 003 employees from all major departments in 1996 and 813 in 2001, generating a cohort of 433 participants in both surveys. Elements of the ergonomic program included employee RSI repetitive strain injury ; training, pro-active assessment of workstations and workstation modifications, and encouragement of early treatment through on-site physiotherapy. Potential risk factors included biomechanical and work organizational aspects of office work. Health status measures included pain intensity and the Work-Disability of the Arm, Shoulder, and Hand DASH ; . Repeat cross-sectional analyses incorporated modifications for shared variance. For the cohort, a Section 19 vol 50.2.
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ACKNOWLEDGMENTS We thank X.-Y. Fu, G. Stark, and R. Desrosiers for providing reagents; B. Xie, C. Spenlehauer, C. Gordon, and S. Kuhmann for helpful discussions; F. Lee and M. Paluch for technical assistance; and C. Nathan for critical review of the manuscript. This work was funded by NIH grants R37 AI36082 and AI43698-03. J.P.M. is an Elizabeth Glaser Scientist of the Pediatric AIDS Foundation and a Stavros S. Niarchos Scholar. The Department of Microbiology and Immunology at the Weill Medical College gratefully acknowledges the support of the William Randolph Hearst Foundation and acetaminophen.
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BAV-3 was isolated from a calf in Japan that exhibited signs of pneumonia, coughing, and serious rhinitis. After plaque purification, the virus was identified as BAV by biophysical characterization and more specifically as BAV-3 by a crossneutralization test. After the virus was propagated in bottle cultures of Madin-Darby bovine kidney MDBK ; cells, aliquots of the virus were kept frozen at 80 C ; until they were used as inoculum. Bovine viral diarrhea virus-free was confirmed by the methods described previously.19, 24 Animals Twelve male Holstein calves were used in this experiment and were divided into four groups Tables 1 and 2 ; . Group 1 consisted of three 7-day-old infected calves calf Nos. 1 3 groups 2 calf Nos. 46 ; and 3 calf Nos. 79 ; consisted of six 1.5-month-old infected calves; and group 4 consisted of three uninfected calves used as controls--one 7-day-old calf No. 10 ; and two 1.5-month-old calf Nos. 11 and 12 ; . All 12 calves had received colostrum within a few hours of birth and had neutralizing antibody against BAV-3 at levels ranging from 1 : 4 64. The nine experimental calves were kept in separate isolation pens throughout the experiment, and the three control calves were likewise kept in similar pens to prevent cross-infection. Experimental procedure Four 1.5-month-old calves calf Nos. 79 and 12 ; were injected intravenously with seven consecutive daily doses of 0.1 mg of DM kg of body weight. On day 5 of DM treatment, three of these calves calf Nos. 79 ; were inoculated.
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Applications in guiding psychological and pharmacologic treatments, but the general consensus is that SPECT and other kinds of neuroimaging are not yet recommended for diagnostic evaluation and treatment monitoring in individual patients. Additional concerns are relevant to the use of neuroimaging in children and adolescents with psychiatric disorders. To date, the overwhelming preponderance of studies have been in adults. PET and SPECT involve exposure to radioactive agents, and MRI and fMRI involve sedation. The long-term effects of exposure of the immature brain to radiation are unknown. Concerns about the investigational uses of brain imaging for children revolve around the unclear risk-benefit ratio of such studies, as well as the difficulties involved in informed consent or assent with regard to a complex technology Hinton, 2002 ; . In a ten-year review published in the Journal of the American Academy of Child and Adolescent Psychiatry in 2000, Hendren and colleagues concluded, citing inconsistencies in data, "Although neuroimaging technology holds great promise for neurodevelopmental research, it is not yet a diagnostic instrument." Hendren et al., 2000 ; This opinion was echoed by Santosh, another review author, who states, "As yet, no specific and consistent abnormality has been detected in childhood psychiatric disorders." Santosh, 2000 ; Even with the continued advances in the understanding of brain structure and function in psychiatric disorders since these reviews, brain imaging has still not progressed to the point of being useful for the clinical diagnosis of these disorders in individual patients. As of this writing, no studies have been published in journals indexed by the National Library of Medicine examining the predictive ability of neuroimaging for psychiatric disorders for either adults or children.
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The frequency at which homologous recombination occurs in plants is low, varies according to crop species, and is proportional to the length of the direct repeated DNA sequence. Recombination between closely linked repeated sequences in maize occurs at frequencies of about 05% Sudupak et al. 1993 ; . Research is underway to identify conditions which would increase the recombination frequency by several orders of magnitude and thus provide a practical means of targeted gene insertion, perhaps without a need for active selection Reiss et al. 1996 ; . 6.4. Recombinase-mediated excision An alternative strategy for marker elimination is the use of site-specific recombinase-mediated marker excision systems. At least four different site-specific recombination systems have been shown to function in plant cells: the bacteriophage P1 Cre lox system Dale and Ow 1990; Odell et al. 1990; Russell et al. 1992 the Saccharomyces cereviseae 2 mm circle FLP FRT system Lyznik et al. 1993; Lloyd and Davis 1994; Kilby et al. 1995; Sonti et al. 1995 the pSR1 system of Zygosaccharomyces rouxii Onouchi et al. 1991; Sugita et al. 2000 ; and the Gin recombinase system of phage Mu Maeser and Kahmann 1991 ; . These have been reviewed by Gilbertson 2003 ; and by Miki and McHugh 2004 ; . All are two-component systems involving a recombinase enzyme, which acts to catalyse recombination between two short, specific DNA sequences. The Cre lox system of marker excision is perhaps best studied, and will be used as an example of excision systems. The Cre lox system consists of the CRE recombinase enzyme, which specifically recognizes and catalyses recombination between two specific DNA sequences lox sequences ; . DNA sequences, including selectable markers, which are flanked by two lox sites, can be precisely excised in the presence of the Cre recombinase Gilbertson 2003; Zhang et al. 2003 ; . The strategy for the elimination of selectable marker genes is thus to produce transformants in which the selectable marker gene is flanked by the specific recombination target sequences and subsequently introduce the recombinase protein. There are three strategies for introduction of the Cre protein. In the first strategy, the inserted DNA can contain the marker gene with an adjacent cre gene, both flanked by a single pair of lox sites. The expression of the cre gene is controlled by an inducible promoter, selected so that the gene remains turned off until particular conditions for example, the introduction of a hormone or chemical to the media ; are met. Secondly, transformants can be constructed which contain the desired trait and a lox-site flanked marker gene. The Cre protein is introduced into selected lead lines by crossbreeding with a line already containing the cre recombinase gene. The marker gene will be excised and, in.
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Angermeier MC. Treatment of facial vascular lesions with intense pulsed light. J Cutan Laser Ther. 1999 Apr; 1 2 ; : 95-100. Abstract Mark K.A.; Sparacio R.M.; Voigt A.; Marenus K.; Sarnoff D.S. Objective and Quantitative Improvement of Rosacea-Associated Erythema After Intense Pulsed Light Treatment. Dermatologic Surgery, June 2003, vol. 29, no. 6, pp. 600-604 5 ; . Christian Raulin, Brbel Greve, Hortensia Grema. IPL technology: A review. Lasers in Surgery and Medicine, Volume 32, Issue 2, 2003. Pages 78-87. Full text link PDF, for example, advil allergy sinus.
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