No medications have been proven to be efficacious in the treatment of the core social or communication impairment seen in autism.
Beyond the Basics Getting Help Most of the arrangements and interactions with vendors that HealthPartners staff will face are covered by the Ethical Considerations, Basic Rules and Examples. This guide was designed to help you work through those common situations. However, we know it is not possible to construct a set of rules or even to anticipate all possible scenarios. If the interaction you are considering appears to meet the Ethical Considerations but is not covered by the Basic Rules, then you should consult with the Corporate Integrity Department. Corporate Integrity staff has worked with a broad spectrum of clinical and business leaders in the organization to develop a detailed set of "Vendor Relations Standards" for the purpose of addressing uncommon or unanticipated vendor interactions. When you contact Corporate Integrity, they will work through the Vendor Relations Standards to help you make a decision that reflects not only the organization's values and responsibilities, but also your own. Corporate Integrity may also consult with the leadership steering group, listed below, to provide guidance on particular issues or types of issues. For more information about vendor relations in general, or for help in working through a particular decision, contact the Corporate Integrity Department. If you wish, you may consult the Vendor Relations Standards directly they are posted on ERIC. However, because this is such a complex and ever-changing area, we believe you will find the expertise of those who are most familiar with the details of the Vendor Relations Standards to be an important resource. Vendor Relations Leadership Steering Group, for example, ovral pills.
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Table 4.10. Echinococcus multilocularis in central Europe: examples of incidence of human alveolar echinococcosis a ; Country and region Switzerland Entire country Entire country Entire country Canton Jura Austria Entire country Germany Bavaria France Franche Comt Doubs Period Number of new Average AE cases b ; per year and periactin, for example, lo ovral emergency.
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Agent by enema if the colon is the focus of the study. You will experience a sense of abdominal fullness and may feel an increasing need to expel the liquid. Be patient the mild discomfort will not last long. Commonly, a contrast agent is injected into a vein to better define the blood vessels and kidneys, and to accentuate the appearance between normal and abnormal tissue in organs like the liver and spleen. Some people report feeling a flush of heat and sometimes a metallic taste in the back of the mouth. These sensations usually disappear within a minute or two. Some people experience a mild itching sensation. If it persists or is accompanied by hives small bumps on the skin ; , the itch can be treated easily with medication. In very rare cases, a patient may become short of breath or experience swelling in the throat or other parts of the body. These can be indications of a more serious reaction to the contrast agent that should be treated promptly, so tell the radiographer immediately if you experience these symptoms. Fortunately, with the safety of the newest contrast agents, these adverse effects are very rare. CT itself causes no pain, though there may be some discomfort from the need to remain still. Who interprets the results and how do I get them? To be filled in A radiologist experienced in CT and other imaging examinations.
E35 * CYTOFLUORIMETRIC ANALYSIS OF LYMPHOCYTIC SUBSETS DURING LONG TERM TREATMENT OF METASTATIC KIDNEY CARCINOMA WITH LOW DOSES OF INTERLEUKINA-2 IL-2 ; AND 5 FLUOROURACILE 5FU ; . CORRELATIONS WITH CLINICAL RESPONSE A. Santagostino, G. Forti, V. Bolis, G. Biaggi, D. Manachino, L. Bellan, S. Lattuada and O. Alabiso * . Unit Operativa di Oncologia Medica, Ospedale S.Andrea, Vercelli e * Cattedra di Oncologia Medica, Universit del Piemonte Orientale "Amedeo Avogadro". The IL-2 is a cytokine that, as a drug, can influence the immunoendocrine network. In our Division we treated, from November 1999 to February 2002, 11 patients with metastatic renal carcinoma with G.O.U.P. protocol which combined low doses of IL-2 with continous infusion of 5FU. During the treatment we have evaluated the immunological effects of this therapy studying the lymphocyte subsets with the cytofluorimetric method. The results obtained with this protocol were the following: overall response 63% 36% of PR and 27% of SD median OS was 16 months and median PFS 13 months. Cytofluorimetric analysis was performed weekly with median follow-up of 26 weeks. The evaluation at the 12th week showed an increment of the absolute value of the CD3 lymphocytes of 27%, while the increase of the CD19 and the NK was respectively 38% and 91%. Furthermore we have evaluated the differences between the responder and non-responder groups. In the table the percent and the absolute value of CD3, CD19 and NK are shown at the beginning of treatment and after 12 weeks. CD3 % W.1 R. Non R. W.12 R. Non R. 69 78 CD3 1756 1636 2334 CD19 187 186 207 NK % 19 14 521 and pioglitazone.
Fat embolism. We have tried to study in detail the diseases which may present as bilateral lower zone lung shadows and to compare the initial diagnosis suspected on the basis of clinical and radiological findings with the final clinico-pathological diagnosis. MATERIAL AND METHODS Fifty six patients admitted in wards of TB & respiratory diseases and General Medicine of J.N. Medical College Hospital, A.M.U., Aligarh were studied. A detailed clinical history and a thorough examination was conducted. Routine haematological.
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Once the addict stops using and begins the recovery process, don't expect that their faults and all the troubles of your shared lives will disappear. You might find, initially, exactly the opposite. Drug use was a coping mechanism for the addict. That coping mechanism will be "raw" for a while, especially when detoxing. Don't expect that a dramatic positive personality change will immediately take place. When a marijuana addict begins going to meetings, there may be interference and conflict with your normal living schedule, routines, and family obligations. This is where your compassion, patience, and encouragement will be called upon. The time spent in the past by the addict in the pursuit of getting and staying high may now be spent going to meetings, reading recovery literature, speaking on the phone with other MA members, writing, meditating, and praying. These activities are of paramount importance to the newly clean addict and your support will be of great value. You may be surprised to find that the addict now insists on attending to certain activities and responsibilities you felt compelled to take care of in the past. This is not a time to condemn past behavior, but an opportunity to practice trust and benevolence. The outcome will be the mutual reward of nurturing a new and healthy relationship. We as individuals can only be responsible for ourselves. This applies to both the addict and the individual who cares. Take each day, one at a time. Be unafraid and happy. Try to adjust yourself to what is, today. Strengthen your own mind and body, exercise your own soul and piracetam!
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DISTRICT OF COLUMBIA HEALTHCARE ALLIANCE BRAND TO GENERIC 3 31 2006 * BRAND NAME KEFLEX 500MG CAP KENALOG IN ORABASE 0.1% PASTE KLONOPIN 0.5MG TAB KLONOPIN 1MG TAB KLONOPIN 2MG TAB KLOR 20MEQ PKT KLORVESS 20MEQ TAB LACRILUBE S.O.P. EYE OINT LAMICTAL 100MG TAB LAMICTAL 25MG TAB LANOXIN 0. 05MG ML ELIXIR LANOXIN 0.125MG TAB LANOXIN 0.25MG TAB LANTUS 100U ML VIAL LASIX 20MG TAB LASIX 40MG TAB LCD 5% IN AQUAPHOR LEDERCILLIN VK 250MG 5ML SUSP LEUKERAN 2MG TAB LEVAQUIN 250 MG TAB LEVAQUIN 500 MG TAB LEVAQUIN 750 MG TAB LEVOTHROID 0.025MG TAB LIBRIUM 10MG CAP LIBRIUM 25MG CAP LIBRIUM 5MG CAP LIDOCAINE 2% VISCOUS SOLUTION LIORESAL 10MG TAB LIPITOR 10MG TAB LIPITOR 20MG TAB LIPITOR 40MG TAB LIPITOR 80MG TAB LO OVRAL-28 TAB LOMOTIL TAB LONITEN 10MG TAB LONITEN 2.5MG TAB LOPRESSOR 50MG TAB LUGOLS SOLUTION MAALOX EXTRA STRENGTH LIQ MACRODANTIN 100MG CAP MACRODANTIN 50MG CAP MAGIC MOUTHWASH SOL MAXIFLOR 0.05% CR MAXIFLOR 0.05% OINT MAXITROL OPTHALMIC DROP MAXZIDE 37.5MG 25MG TAB MEDROL 4MG DOSEPAK MEDROL 4MG TAB MEGACE 40MG TAB MEGACE 40MG ML ORAL SUSP GENERIC NAME CEPHALEXIN 500MG CAP TRIAMCINOLONE 0.1% PASTE CLONAZEPAM 0.5MG TAB CLONAZEPAM 1MG TAB CLONAZEPAM 2MG TAB POTASSIUM CHLORIDE 20MEQ POTASSIUM CHLORIDE 20MEQ LACRILUBE EYE OINT LAMOTRIGINE 100MG TAB LAMOTRIGINE 25MG TAB DIGOXIN 0.05MG ML ELIXIR DIGOXIN 0.125MG TAB DIGOXIN 0.25MG TAB INSULIN GLARGINE 100U ML VL FUROSEMIDE 20MG TAB FUROSEMIDE 40MG TAB LCD 5% IN AQUAPHOR PENICILLIN VK 250MG 5ML CHLORAMBUCIL 2MG TAB LEVOFLOXACIN 250MG TAB LEVOFLOXACIN 250MG TAB LEVOFLOXACIN 250MG TAB LEVOTHYROXINE 0.025MG TAB CHLORDIAZEPOXIDE 10MG CAP CHLORDIAZEPOXIDE 25MG CAP CHLORDIAZEPOXIDE 5MG CAP LIDOCAINE 2% VISCOUS SOL BACLOFEN 10MG TAB ATORVASTATIN 10MG TAB ATORVASTATIN 20MG TAB ATORVASTATIN 40MG TAB ATORVASTATIN 80MG TAB LO OVRAL-28 TAB DIPHENOXYLATE ATROPINE TAB MINOXIDIL 10MG TAB MINOXIDIL 2.5MG TAB METOPROLOL 50MG TAB LUGOLS SOLUTION MAALOX EXTRA STRENGTH NITROFURANTOIN 100MG CAP NITROFURANTOIN 50MG CAP MAGIC MOUTHWASH SOL DIFLORASONE 0.05% CREAM DIFLORASONE 0.05% OINT MAXITROL OPTHALMIC DROP TRIAMTERENE 37.5 HCTZ 25 METHYLPREDNISOLONE 4MG DOSE METHYLPREDNISOLONE 4MG TAB MEGESTROL 40MG TAB MEGESTROL 40MG ML ORAL SUSP PAGE 10 27 17 and pletal.
Note: When an implantable intrathecal infusion pump is determined to be medically necessary, the supplies necessary for the proper use of the pump are considered medically necessary. Investigational Not Medically Necessary: Implantable infusion pumps are considered investigational not medically necessary for the infusion of heparins for thromboembolic disease or antibiotics for osteomyelitis. All other uses of implantable infusion pumps, including fully implantable insulin pumps, are considered investigational not medically necessary. Rationale The role of opioid therapy in treatment of pain is well established in the medical literature. Individuals who have proven unresponsive to less invasive medical therapy and who require large doses of opioids may be candidates for an implantable delivery system that permits intrathecal administration. This system delivers the opioid directly to the receptors in the spinal cord, allowing smaller doses to be used and thereby minimizing side effects. This position is supported by multiple case control studies. The use of continuous chemotherapy infusion treatment has been studied for patients with certain types of cancers, including, but not limited to, primary hepatic cancer, metastatic colorectal cancer to the liver, and various head and neck cancers. This method of chemotherapy infusion has been found to improve medical outcomes in select individuals where continuous chemotherapy is believed to be appropriate. The evidence supporting this conclusion includes multiple randomized controlled trials. Prospective randomized trials of individuals with unresectable liver disease have shown that compared to conventional systemic therapy, hepatic artery infusion is associated with an increased tumor response rate. Implantable pumps for delivery of medication to the intrathecal space have been developed as an alternative to chronic systemic administration for the treatment of spasticity of cerebral or spinal origin. These pumps have been demonstrated in numerous randomized controlled trials to reduce adverse effects such as tolerance, dependency, and neurotoxicity. The use of implantable pumps for infusion of antithrombotic medications for thromboembolic disease, or for the infusion of antibiotics for osteomyelitis, has not been demonstrated to provide any additional improvement in net health outcomes above standard care with bolus or subcutaneous drug administrations. This therapy does not prevent the occurrence of complications or morbidity nor does it significantly relieve pain over other less invasive treatment methods. The risks involved in the implantation and maintenance of, because ovral 21 side effects.
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Antibody HuM195 could produce PCR negativity in patients in hematologic CR.6 This seemed logical enough since a high proportion of patients with APL are not only CD33 positive, but that APL cells are rich in CD33. Accordingly, we administered mylotarg an anti CD33 antibody conjugated to the cytotoxic agent calicheamicin ; together with ATRA, 7 treating 11 lowrisk patients and 7 high-risk patients. Once in CR patients were to receive 7 courses of mylotarg at the induction dose of 9 mg m2 on day1 + ATRA, on a 2 weeks on- 2 weeks off schedule. As in the L-ATRA trial, PCR testing was done every 3 months for 1-2 years, with idarubicin added for molecular, as well as hematologic extramedullary failure. All 11 low risk patients entered CR, and 9 remain alive in CR with a median follow-up of 137 weeks. Mylotarg was tolerated as idarubicin; thus, there was no more difficulty in administering the planned number of post-remission course than with idarubicin. Comparison of event free survival in low-risk patients with that seen in the historical patients described above revealed superiority for mylotarg + ATRA. Although conventional statistical significance was not reached and recalling that the historical patients did not receive ATRA in CR but were treated for 2 years ; , we nonetheless believed that it was very unlikely that mylotarg + ATRA was inferior to idarubicin + ATRA. This view together with the success reported by Lo Coco et al.8 with mylotarg in treatment of molecular relapse prompted us to adopt this drug as our standard chemotherapeutic agent in APL. ATRA + ATO + - Mylotarg in APL: General Considerations Reports from India9 and Iran10 have noted the effectiveness of ATO in untreated APL. Of particular interest to us is Shen et al's trial 4 randomizing untreated patients to ATRA, ATO, or ATRA + ATO. In remission patients continued to receive the originally assigned arm but were also given chemotherapy. The greatest reduction in the level of pre-treatment PML-RAR transcripts at time of CR occurred in patients given ATRA + ATO. Given that addition of ATRA to ATO did not increase toxicity, it appears that the optimal nonchemotherapy regimen to investigate in untreated patients is ATRA + ATO. Our trial differs from the Chinese trial as follows: 1 low-risk patients do not receive chemotherapy mylotarg ; unless 2 consecutive PCR tests performed 2-4 weeks apart at a sensitivity level of10-4 beginning 3 months from CR date are positive2 high-risk patients receive mylotarg 9 mg m2 on day 1 of treatment , but thereafter are managed identically to low-risk patients, and3 ATO begins 11 days after the first dose of ATRA rather than simultaneously. The decision to give mylotarg to high-risk patients reflected the poor results with L-ATRA monotherapy in such patients, as described above. Administration of ATO was delayed to prevent hepatotoxcity; none has been seen however and a revised version of the protocol would call for ATRA and ATO to begin concurrently. Is it ethical to use ATRA + ATO, while reserving chemotherapy in low-risk patients given that the cure rate of such patients is 90% with standard ATRA + idarubicin? An answer to this question requires consideration of the ATRA + ATO trial's statistical design. Thus, the design operates such that if the true CR rate with ATRA + ATO is only 60%, rather than our historical 80%, the expected median sample size is 18. However, given our accrual rate, 21 patients would be expected to have received ATRA + ATO by the time accrual is stopped. 60% of these 21 would get a CR compared to 80% of the 21 with historical treatment. Thus the cost of conducting the trial is 80%21 ; 60% 21 ; 4 patients. If the true CR rate with ATRA + ATO is below 60% the trial is designed to stop earlier, leading to the same cost. Whether this potenHaematologica Reports 2005; 1 7 ; : September 2005.
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Supported by grants from the Fondo de Investigaciones Sanitarias de la Seguridad Social Spanish Ministry of Health ; . 2 Correspondence: FAX: 34958249015; e-mail: engarcia ugr.
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Any illicit drugs past month 12% 15% 12-19% Top fifth Any drug other than 6% 7% 5-10% Top fifth marijuana Marijuana use ever 40% 50% 45-55% Marijuana use past month 22% 31% 26-36% Top fifth Inhalants 12% 13% 11-15% Cocaine use 9% 10% 8-12% Top fifth Ecstasy use ever 11% 10% 8-12% Heroin use ever 3% 2% 1-3% Alcohol use past month 45% 47% 45-52% Third fifth Binge drinking past month11 28% 31% 26-36% Second fifth Any drug or alcohol 9% 12% 10-16% Top fifth dependence or abuse Needing but not receiving 6% 8% * 6-11% Top fifth * alcohol treatment Needing but not receiving 5% 6% * 5-10% Top fifth * drug treatment Blanks indicate the survey does not report on this particular question. * These rankings do not take into account treatment provided by clinicians who operate independently of treatment facilities, or treatment provided in hospital outpatient facilities, in all states and provera.
The Department currently limits the amounts of drugs the Medicaid Program will pay for. These limits apply generally to all drugs prescribed to Medicaid clients. Additional limits are placed on drugs the Department has chosen to target.
ILLINOIS REGISTER DEPARTMENT OF PUBLIC HEALTH DRAFT NOTICE OF ADOPTED AMENDMENTS Section 545.Appendix C Emergency Contraception Protocols CONTRACEPTIVE INTERVENTION SAMPLE PROTOCOL I A. GENERAL Each survivor of sexual assault will receive medically and factually accurate written and oral information about emergency contraception as soon as possible and, in any event, no later than 12 hours after the sexual assault survivor presents herself himself at the hospital for emergency treatment services; the indications and counter-indications and risks associated with the use of emergency contraception; and a description of how and when alleged survivors will be provided emergency contraception upon the written order of a physician licensed to practice medicine in all its branches. If the alleged sexual assault survivor accepts this treatment, the physician will administer emergency contraception as approved by the federal Food and Drug Administration FDA ; unless contraindicated for medical reasons while the alleged survivor is in the emergency room. Each survivor of sexual assault will be provided with an appropriate referral to a physician licensed to practice medicine in all its branches as provided in the Medical Practice Act of 1987. CONTRACEPTIVE INTERVENTION SAMPLE PROTOCOL II CATHOLIC HOSPITAL ASSOCIATION ; A. GENERAL Each survivor of sexual assault will receive medically and factually accurate written and oral information about emergency contraception as soon as possible and, in any event, no later than 12 hours after the sexual assault survivor presents herself himself at the hospital for emergency treatment services; the indications and counter-indications and risks associated with the use of emergency contraception; and a description of how and when survivors will be provided emergency contraception upon the written order of a physician licensed to practice medicine in all its branches. A female survivor of alleged sexual assault who shows a negative result for pregnancy on the blood test and a negative result with respect to the urine dip-stick test, and whose history corresponds to this, will be offered a contraceptive intervention of high dose Ofral or equivalent ; . If the alleged sexual assault survivor accepts this treatment, the first dose will be provide in the emergency department to achieve the contraceptive effect. If the alleged survivor presents a positive result on the tests, the survivor will be counseled that the emergency department will not offer the formulation. If the blood test is positive for pregnancy, the alleged sexual assault survivor will be counseled that this pregnancy is not of immediate or recent origin. If the urine test is positive and relates to the individual's history, it indicates that the LH surge is under way or that the woman is ovulating, and that a contraceptive formulation would not be effective in preventing ovulation, and contraceptive intervention will.
| Min ivral side effectsTable 1. Baseline characteristics according to Pro12Ala genotype of type 2 diabetes patients randomized to 1 or mg day of dual PPAR agonist treatment. Variable Pro12Pro Pro12Ala Ala12Ala P-value Baseline n 278 ; n 59 ; n Weight kg ; 94.3 19.6 ; 95.3 17.3 ; 93.7 26.6 ; NS BMI 32.6 5.5 ; 33.6 5.3 ; 30.8 7.2 ; NS Hba1C % ; 8.0 1.2 ; 8.1 1.2 ; 9.0 1.7 ; NS F-P-gluc mM ; 9.6 2.4 ; 10.3 3.1 ; 10.9 2.6 ; 0.01 S-insulin ; 103.1 80.7 ; 127.0 183.3 ; 80.6 52.6 ; NS Trig. mM ; 2.2 1.2 ; 2.2 1.3 ; 1.6 0.8 ; NS Cholest. mM ; 5.1 0.9 ; 5.1 0.8 ; 5.0 0.9 ; NS BMI, body mass index; DiaBP, diastolic blood pressure; HbA1C, glycosylated hemoglobin; F-P-glucose, fasting plasma glucose; S-insulin, serum insulin; Trig, triglycerides; Cholest, total cholesterol.
FIRST TRIMESTER Pre-emptive abortion: endometrial aspiration ; performed prior to 5-6 wks LMP when pregnancy cannot yet be verified by pelvic exam syringe + cannula suctions out uterine lining + fetal & placental tissue local rarely used b c cervical dilatation not req'd slightly chance of missed abortion since so small an amt of tissue Early uterine evacuation pelvic exam confirms pregnancy larger cannula cf. pre-emptive 5-6mm ; Vacuum aspiration: need to dilate cervix for larger instruments ie as electrically powered aspirator SECOND TRIMESTER Dilatation and curettage D & C ; at 12-16 wks LMP standard gyne procedure to rx XS bleeding, dx uterine problems under general anesthetic Dilation and evacuation D & E ; 12 wks LMP fetal tissue is larger, uterus is softer and easier to injure cf 1st trimester and req. more skills.-U S, Cervical dilation necessary for larger instruments: place 1 or more laminaria sticks sterilized seaweed that absorbs moisture and expands ; into cervix the day before; remove at time of abortion; can also use PG which softens cervix by incr. proteoglycans and changing the biophysical properties of collagen. Forceps, curette, vacuum suction oxytocin helps uterus contract and thus slows bleeding Induction abortion injection of solution through abdomen into amniotic sac or IM the solution may be: a ; saline lower complic rate and incomplete abortion rate, but longer wait until labour, slight risk of serious emergency ; b ; PGF2 works more quickly cf saline, less emergency risk, but more N V, diarrhea, higher failure rate of 1st installation, retained placenta, more painful contractions, . cervical tearing risk ; c ; saline + PG + urea this method not used until 16 wks LMP b c prior to this time, the sac is not large enough to be located 8 to 24 hours later -- contractions - dilatation -- expulsion then do D & C remove any remaining tissue In hospital approx.48 PG suppositories inserted into vagina - contractions - expulsion newest, least known method usu. used when fetus has died and woman is not going into labour takes about 90 hours. + N V. diarrhea. Fever Hysterectomy removal of fetus + placenta through serious complications incision into abd. and uterus like a small C-section ; RU 486 mifepristone, aka the abortion pill ; antiprogesterone medication, most effective when used within 9 weeks of the LMP and when followed 48h after ingestion of a PG; 96% success rate approved for use in France, but not in US or Canada a very controversial issue ; Morning After Pill blocks the release of progesterone and affects the uterine lining so that even if an egg is fertilized, it can't implant a strong birth control pill Ovvral ; is used; 2 tablets are taken right away and 2 tablets 12 hours later; vomiting may occur and 2 more may have to be taken if they were not digested 3. the potential complications of the procedure for lst trimester, complications occur 1% of the time; these incr. the later the pregnancy symptoms and signs will generally appear a few days after the abortion a ; Infection fever 100.5 F, bad cramping, foul-smelling vag. dischg ; . rx: tetracyclin ampicillin; if unrx: illness, sterility, death b ; Retained tissue clue: bleeding, passage of clots, bleeding 3wks, signs of pregnancy lwk ; Likely to become infected rx: methergine egotrate: to stim. uterine contractions aspiration c ; Perforation: more risk in D&E.
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An increased accumulation of fat after carbontetrachloride CC14 ; injury in the livers of Enovid-treated compared to untreated control female rats has been reported Joshi and Rao, 1969 ; . However, the recovery of the liver following CCl4 injury though delayed by Enovid was found to be complete. In another study on female rhesus monkeys, it was observed that the increase in serum enzymes of hepatic origin seen after the administration of aflatoxin a powerful hepatocarcinogen could be prevented by the simultaneous administration of ovulen an oral contraceptive agent OCA ; Belavady et al., 1973 ; . These results were supported by the work of Mg bodile and Holscher 1976 ; , who found that the pre-administration of 100 g ovral-28 for 16 days mitigated the acute effects of a single dose of aflatoxin B1 in rat liver. Studies of Mukundan et al. 1981 ; show that daily administration of 5 g ethinylestradiol, 100 g ethynodiol diacetate in the form of OCA-ovulen-50 to female rats leads to an increase in liver lipids and mitostimulation in a nonregenerating liver, but mitoinhibition in liver regenerating after partial hepatectomy pH ; . We have recently shown that after similar treatment, there is a slight increase in the level of the tumour marker enzyme -glutamyl transpeptidase GGT ; and increased agglutinability of isolated hepatocytes with concanavalin A Con A ; suggesting certain degree of liver damage and or transformation in OCA-treated female rats Annapurna et al, 1987 ; . Inhibition of cell proliferation and or DNA synthesis is also brought about by many carcinogens and this is considered to have some significance in the carcinogenic process Farber, 1976 ; . The studies reported in this paper were designed to find out if established tumour initiator and promoter compounds like diethylnitrosamine DEN ; and phenobarbital PB ; respectively would behave like OCA with regard to the above cited effects, when administered singly and in combination over a short duration of 7 weeks. Experimental Ovulen-50 was obtained from Searle Company, Bombay. DEN from Sigma Chemicals, St. Louis, Missouri, USA, and phenobarbital from Indian Drugs and Pharmaceuticals, Hyderabad. [3H] Thymidine was obtained from Bhabha Atomic Research Centre, Bombay. All other compounds used were of the highest purity available. Two experiments were carried out. In the first experiment 64 weanling female rats of Wistar NIN strain were divided into two equal groups. One group received a single intraperitoneal injection of DEN 200 mg kg body weight ; in saline initiated rats ; . The other group received the same quantity of saline and served as uninitiated control. Four weeks after this injection, 16 rats from DEN-initiated group and 16 from saline-treated group received either propylene glycol or one-tenth tablet of the OCA-ovulen-50 as propylene glycol suspension for 7 weeks. Three or four rats from each of the four treatment groups-- i ; control, ii ; DEN, iii ; OCA, iv ; DEN + OCAtreated were sacrificed at 0, 24, 48 and 72 h after pH. pH was performed by removing two third of the liver under light ether anesthesia Higgins and Anderson, 1931 ; . One hour before sacrificing, 5 Ci of [3H]thymidine sp. act. 98 Ci mmol ; was injected through the tail vein. After sacrificing the rats, the livers were rapidly removed and examined for total protein, DNA, RNA and [3H]thymidine incorporation into DNA by the method described earlier Mukundan et al., 1981.
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